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After 48 h, biofilms were washed once with 1 PBS followed by treatment with 30 mM GSH (pH 7 0.2) and 30 mM GSH (pH 7 0.2) + 4 g/mL amikacin and also with 30 mM GSSG and 30 mM GSSG + 4 g/mL amikacin for 24 h, at 37C and 150 rpm