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Vitamin B1 (Thiamine): Helps convert carbohydrates and fats into energy

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Convert vial amount to micrograms (if needed): mg 1,000 = mcg Concentration: total amount (mcg) total liquid (mL) = mcg/mL Draw volume: target amount (mcg) concentration (mcg/mL) = mL U100 units: mL 100 = syringe units Worked example (math only) If a vial is labelled 5 mg (5,000 mcg) and you add 2 mL, the concentration is 2,500 mcg/mL

All housing, handling, and experimentation that involved the rats were conducted following the procedures set in place by the National Institutes of Health guide for the care and usage of laboratory animals [2]

Tandon et al., 2022