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Often used in pulsatile research protocols

Monolayers were infected with either Ad-GFP or Ad-Cre (100 MOI) and efficiency of the IR deletion was verified by measuring IR mRNA and protein expression 48 h after infection (Figure 3a and 3b)

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45 Thus, since the 1990s, in vitro-synthesized mRNA has been increasingly applied for protein replacement in preventive and therapeutic vaccines