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The remaining reagents were purchased from Euroclone S.P.A
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Next, 40 mL of seed culture grown overnight was added to 4 L of LB broth and incubated at 37 C until it reached an optical density of 0.6 as measured at 600 nm ( A 600 )
The presence of --carotene, --cryptoxanthin, lutein, and violaxanthin in spinach also increases carotenoid levels in lipoproteins
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