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Glutathione is converted into a fine mist by the nebulizer and inhaled directly into the lungs where it is rapidly absorbed into the bloodstream

Pyroglutamate is a glutamate precursor whose CSF concentration is 120 M (58), rivaling the extracellular glutamine concentration of 400 M (basal glutamate is 2-3 M)

Simm, Redox Biology Role of advanced glycation end products in cellular signaling, Redox Biol

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Both media were supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 2 mM glutamine (25030081), all from ThermoFisher Scientific, plus 100 units/mL penicillin (Laboratorios ERN.S.A., Madrid, Spain