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After three membrane washing steps, a diluted secondary antibody solution was introduced and incubated at room temperature for 2 hours to detect the presence of specific reaction products

Note: If you experience a severe allergic reaction, such as swelling of the lips, face, or tongue, trouble breathing, or widespread hives, call 911 or go to the nearest emergency room immediately

Materials and methods Cell culture and reagents Rat renal proximal tubular cells (RTCs, NRK-52E) were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan), and culture cells in Dulbeccos modified Eagles medium (DMEM) were supplemented with an antibiotic/antifungal solution and 5% fetal bovine serum (FBS, pH 7.2)

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