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NAPQI binding and subsequent superoxide generation are initially associated with a modest drop in voltage potential across the inner membrane without an increase in oxygen consumption or proton leak i.e., without uncoupling of oxidative phosphorylation

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The impact of this insight was further extended by the finding that the LASSS-treated HGF (after the wash-out of un-reacted LASSS) exhibited nitration resistance to the optimal peroxynitrite-treatment (at the same 1:2000 ratio as before) as shown in Figure 2b

Authors contributions Study concept and design (GYW), drafting of the manuscript (EMY, GYW), literature review and writing of the manuscript (EMY), critical revision of the manuscript for important intellectual content (GYW), manuscript proofreading (GYW, EMY)

The safety review 13 of secretagogues notes that long-term data is limited, so vigilance is essential