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This was due to the presence of autoantibodies against folate receptor which blocked the folate binding site

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Injection Site Reactions : Some people may have injection site reactions, such as redness, swelling, or itching at the injection site

IF Staining of mouse liver using 66323-2-Ig Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)