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Details of the antibodies and assay given in supporting information file

Four m thick sections were obtained and stained with hematoxylin and eosin (H & E) and Perls Prussian blue stains

This positions postbiotics as context modulators that complement established lean masspreserving interventions rather than substitutes for them ( Although postbiotics are not expected to exert direct anabolic effects on skeletal muscle, their potential relevance to lean mass preservation lies in indirect metabolic pathways

Statistical Analysis In line with EMA, FDA and PMDA guidelines, a crossover design was chosen to leverage the lower within-participant variability in PK measures and thereby limit the number of participants required for the study [28,29,30]

The mean baseline body weight was 91.9 kg and 91.3 kg in the semaglutide tablets 14 mg and empagliflozin 25 mg arms, respectively