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It is also important to mention that RNA and protein levels were determined by real-time PCR and Western blotting, respectively
Through this immune activation in T cells, interferon gamma is essential in the fight against infections
B12 deficiency can lead to severe and potentially irreversible neurological damage if misdiagnosed or inadequately treated
Continuous ROS production results in oncogene and tumor suppressor gene changes, as well as chromosomal abnormalities by oxidative genome damage, which includes the oxidation of guanine to form 8-OhdG and 8-oxo,7,8-dihydroguanosine (8-OHG) in RNA and DNA (100)
While our qPCR analysis relied on a single reference gene (GAPDH) that showed stable expression, future studies could benefit from incorporating multiple reference genes as recommended by the MIQE guidelines [51]
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