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Antibodies were diluted in a blocking solution
The formulation, enclosed in liposomes, ensures efficient nutrient absorption into the bloodstream
Autoradiography or the Odyssey Imaging System (LiCor Biosciences, Lincoln, NE) was used to visualize protein bands
A comprehensive panel of antibodies was utilized for Western blotting, targeting phosphorylated and total forms of ERK1/2, p38 MAPK, JNK1/2, and NF-B p65 (Ser536), with -actin serving as a loading control