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RNA extraction, cDNA synthesis and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a TRI Reagent kit (Sigma) according to the manufacturers instructions

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(2013) proposed new criteria in hopes of providing a better prognostic tool to measure risk and guide care

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A perfect dosage for first visible results from the first month

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www.jaoa.org/content/111/2_suppl_1/eS10.full

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