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Tetrahydrofuran was distilled over sodium benzophenone ketyl and CH 2 Cl 2 was distilled over phosphorus pentoxide
Methods Materials Diamide, dithiothreitol (DTT), ethylenediaminetetraacetic acid (EDTA), GSH, GSSG, and yeast GR were from Sigma-Aldrich, l-cysteine was from Carl Roth, isopropyl--d-1-thiogalactopyranoside was from Serva, methyl ether poly(ethylene glycol) 24 maleimide (mmPEG) was from Iris Biotech, and NADPH was from Gerbu
World J Eng Technol
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