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Additionally, to obtain data on the tissue expression of up- or downregulated protein in amyloid PET-positive and -negative participants, tissue expression data was downloaded from the human protein atlas 34

Quantitative real-time PCR (qRT-PCR) analysis Total RNA was isolated from cortical samples by using RNAiso plus (Takara, Shiga, Japan) according to the manufacturers instructions

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Schulz TJ, Zarse K, Voigt A, Urban N, Birringer M, Ristow M: Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress

This short phase IIa IMODALS trial, as a prelude to the phase IIb/III MIROCALS trial, showed that low-dose IL-2 (aldesleukin) produces a robust upregulation of the peripheral T reg cell count and a lowering of at least one inflammatory mediator (MCP-1/CCL2)