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Blood samples in EDTA (for all analyses except glucagon, GLP-1 and glicentin) or P800 tubes (Becton Dickinson, Franklin Lakes, NJ) were immediately placed on ice and centrifuged at 4C and 2500g for 10 minutes, after which plasma was aliquoted and stored at -80C until analysis

For any questions regarding medical treatment, consult your doctor.

others require continuous administration

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