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Heterologous expression of an engineered bifunctional bacterial GSH biosynthetic enzyme (GshF) in mitochondria enables mitochondrial GSH production and ameliorates the metabolic and proliferative defects caused by its depletion
Mouse monoclonal antibody against Flag tag (#F3165), and rabbit polyclonal antibodies recognizing GLUT1 pS226 (#ABN991), nickel affinity gel (#P6611), anti-Flag M2 affinity gel (#A2220), 2-deoxy-D-glucose (2-DG) (#D8375), palmitic acid (#P0500), dithiothreitol (#D9779), ethylenediaminetetraacetic acid (EDTA) (#E6758), phenylmethanesulfonyl fluoride (PMSF) (#P7626), leupeptin (#L2884), 2-Bromohexadecanoic acid (2-BP) (#21604), hydroxylamine (#159417), palmostatin B (#178501), N-Ethylmaleimide (NEM) (#04260), mPEG-maleimide 5 kDa (#63187), TCEP (#646547), crystal violet (#C0775), and IPTG were purchased from Sigma
ATG2A, autophagy-related protein 2 homolog A
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