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For the in vitro experiments, a murine macrophage cell line RAW 264.7 was used
Zhang T, An Y, Shen Z, et al
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it has been the subject of extensive research for decades
Also, metabolites of VPA -oxidation (including propionic derivatives and 4-en-VPA) inhibit the mitochondrial CPS I, which is the first enzyme necessary for ammonia elimination via the urea cycle in the liver [19, 24]
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