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While this is a necessary first step, it frequently creates highly reactive, electrophilic intermediate metabolites that are actually more toxic and dangerous than the original parent compound

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This creates a dangerous cycle: toxin buildup increases oxidative stress, which further depletes glutathione, making detoxification even less efficient

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C O Activated Thiol Sepharose 4B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides