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Studies in animal models with gut microbiota depletion have shown increased plasma tryptophan, elevated brain serotonin concentrations, and reduced kynurenine pathway activity, all of which normalize following microbiota restoration (229231)

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For purification, a 5 ml GSTrap FF (GE Healthcare) was equilibrated with at least five column volumes of reducing lysis buffer

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Exactly how much lighter depends on your starting weight, but people with obesity can expect about 3% body weight reduction, on average, by this point