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However, it is important to note that samples may have to be produced for submission of product registration

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The fresh sample was extracted from 10% TCA solution, centrifuged 10,000 g , for 10 min under cold condition and the supernatant was incubated in an assay mixture [0.2 M phosphate buffer, 15 mM dithiobis-(2-nitrobenzoic acid)] and diluted with water for 30 min