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The ago-allosteric small molecule compound 2 enhances binding of the agonist GLP-1 but does not affect binding of the antagonist exendin (9-39) and it cannot be inhibited by the peptide antagonist exendin (9-39), which is not surprising considering the intracellular binding site of compound 2 32,35,36
The BlandAltman plot was used to compare the compatibility of the modified GPx-DTNB and original GPx-DTNB methods, evaluate the agreement between the two methods, assess method parameters, and verify the normality of the observed differences
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