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A volume of 4-NPG (final concentration of 1 mM) was added to the reaction and decrease in absorbance at 305 nm was recorded on an Envision multilabel plate reader (Perkin Elmer)
It is the rare drug (some types of tetracycline) that become toxic if outdated
She declined to answer further questions, but said authors are preparing a reply letter in defense of their study to submit to the journal
[PMID: 6708590] PubMed record related to this compound for laboratory literature review
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