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Develop a rotation pattern
Cryo-EM grid preparation For grid preparation, 3 uL of 3 mg/mL purified OCTN2 was applied to a freshly glow discharged holey gold grid, with UltraAuFoil R0.6/1, 300 mesh used for inward-facing, and UltrAuFoil R1.2/1.3 300 mesh grids used for OCTN2 occluded and OCTN2 ipratropium
Let steep until cool or as long as overnight
One more important methodological problem is that blood leukocytes are highly heterogeneous cell population which includes various cell types, such as lymphocytes, monocytes, granulocytes, etc
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