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According to the Mayo Clinic, most people consume adequate amounts of B vitamins through their diet, though the elderly, particularly those with pernicious anemia, athletes, strict vegetarians, and people who consume a lot of alcohol may be deficient in the B vitamins and may require injections or oral supplementation of B vitamins or vitamin B-12, in particular
One study found that even up to 2,000 micrograms a day of methylcobalamin wasnt enough to correct vitamin B12 deficiency in one of three vegans tested
Avoid repeated freezethaw cycles and discard any unused material in accordance with institutional waste procedures
A 2003 J Natl Cancer Inst paper by Cha et al