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It is endogenously synthesized in our bodies from essential amino acids lysine and methionine

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Methods Animals Twelve-weeks-old male C57BL/6 mice were housed in the animal facility of the Department of Pharmacology and Pharmacotherapy, University of Pcs, in a room with controlled temperature and humidity, in standard polycarbonate cages (365 mm x 207 mm x 144 mm) at 12-hour light-dark cycles (lights on: 6 a.m.)