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Briefly, ~0.4 kb DNA upstream (5 flank) of the open reading frame (ORF) was amplified by PCR of genomic DNA using primers P1 and P2 (Supplementary Table 1 and Supplementary Fig

However, in oxidoreductases proteins, such as TRXs and glutaredoxins (GRXs) this pKa is lower, because it is affected by the surrounding micro-environment (Mailloux et al., 2014)

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