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Cell pellets were collected and lysed in 9 M urea or RIPA buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% Na deoxycholate, 0.1% SDS, and 1% Triton X)
The resulting elimination half-life of approximately 6 days supports convenient once-weekly subcutaneous dosing
Acta 124 MillerW
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