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Plates were incubated at RT for 1 h and the fluorescence intensity measured at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer)
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These include nausea, vomiting, diarrhea, and constipation
To annotate the neuronal cell populations, clustering was run at a resolution of 1, and clusters were merged and labeled based on the expression of markers of key neurotransmitters ( Slc32a1 , Gad2 , Slc17a6 , Chat , Dbh , Th , and Tph2 )
This is precisely why our program (physician-led by Dr
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