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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

Each batch undergoes independent analytical testing to verify: Compound identity Assay accuracy Purity confirmation Microbial safety Endotoxin levels Heavy metal screening Full batch traceability Research Use Limitation Used solely for in vitro experiments Not permitted for clinical trials involving humans Not permitted for human or veterinary administration Not permitted for investigational human use Independently analysed by Liquilabs s.r.o

262, Berk, Michael, et al

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