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While density for the N-terminal lipidation was not visible in the lower resolution cryo-EM map of the repeat Cagri-CTR-Gs complex ( N = 2), the outward rotamer of H296 ECL2 was observed, congruent with similar interactions between the N-terminal acylation and ECL2

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The effect plateaued by week 12, suggesting either receptor saturation or compensatory downregulationa detail with protocol implications for researchers designing extended-duration studies