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R471C has been shown to localise to the membrane and has ~15% activity of wildtype OCTN2 in CHO cells, which is consistent with the observed reduction in currents for R471A, meanwhile Y358N in CHO cells has ~8% activity, though localisation data for Y358N is unavailable 11,50

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After verifying the low levels of GSH in brain, 2D electrophoresis was performed to detect oxidized protein carbonyls which further confirm GCL knockdown causes protein oxidation in vivo

This blanching response happens because pressing temporarily pushes blood away from the area