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Effect of reduced glutathione, water soluble vitamin E analogue and butylated hydroxytoluene on the post thaw characteristics of boar spermatozoa

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An overnight culture was used to inoculate 4 500 mL of LB medium containing 100 g ml 1 ampicillin (pETM20- msadh ) or 50 g ml 1 kanamycin (pETM11- msadh pETM11- mdh ), respectively

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Remove drops from fridge