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Optimization of anesthesia protocol for resting-state fMRI in mice based on differential effects of anesthetics on functional connectivity patterns
Researchers most commonly select 20mg for: Single-arm pilot studies baseline characterisation of triple-agonist activity in a chosen cell or tissue model Receptor binding assays GLP-1R, GIPR, and glucagon receptor displacement work where modest substrate quantities suffice Comparative studies when R3TA is benchmarked head-to-head against single- or dual-receptor agonists (semaglutide, tirzepatide) in matched assays Short in-vitro time-courses 12 week protocols where 20mg covers full study consumption without surplus For dose-response curves, extended multi-arm studies, or higher-throughput assay panels, the R3TA 30mg preparation is the more cost-effective and protocol-appropriate size
Aug 2013;24(4):255-63
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Srenson's thinking was coloured by the fact that a previous drug, Redux, approved in 1996 by the FDA for weight loss and launched with great fanfare, had to be withdrawn a year later after it was found to cause major heart attacks and to be largely ineffective
