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The fraction of mitochondria containing ER-associated membranes was then purified using the mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to the manufacturers protocol

In LOsG-induced prediabetes, although pancreatic ROS stress remained high on LOsG treatment day 38, there were no alterations in mRNA levels of ER stress (XBP1 and BIP), inflammation (IL6 and IL1), and hypoxia-responsive (HIF1 and GAPDH) genes between LOsG- and sham-treated groups

Weight and metabolic changes generally develop over several weeks to months

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