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Santaella ML, Font I, Disdier OM
F.TheodorakisM.et al (2011)
Commit to a Process for Lasting Benefits : Real changes from this therapy, like improved muscle tone, better sleep, and enhanced fat loss, appear gradually over several weeks
The flow-through was collected the next day and concentrated with a Amicon Ultra centrifugal filter (molecular weight cut-off of 100 kDa, Millipore) and then subjected to a Superdex 200 Increase 10/300GL column (Cytiva) with running buffer (pH 7.5) containing 20 mM HEPES, 100 mM NaCl, 10 mM MgCl 2 , 100 M TCEP, 10 M ligand, 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN and 0.0002% (w/v) CHS
L-Carnitine + B -
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