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Materials and methods Reagents and antibodies The following antibodies were used for western blotting: anti-NLRP3(15101S, Cell Signaling Technology), anti-GSDMD (NBP2-33422, Novus Biologicals), anti-IL-1(ab234437, Abcam), anti-IL-18 (ab207323, Abcam), anti-CD31(AP3628, R&D systems), anti-VEGF (sc-7269, Santa Cruz), anti-CCL11(ab133604, Abcam), anti--actin (4970S, Cell Signaling Technology)

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Because regulations in this area continue to change, the safest approach is to work with a physician who keeps up with current rules rather than ordering from an online source making broad claims

Reprod Sci

[11] Micke P, Beeh KM, Schlaak JF, Buhl R