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Thus, there is an urgent need to investigate and compare the expression of GPX1 in persisting beta cells and other islet cell-types at and after diagnosis of T1D with non-diabetic cases with and without islet cell autoantibodies

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The above cycle is repeated until the desired sequence has been synthesized Optionally, the N -terminal amino group undergoes capping, thereby preventing residual unreacted resin-bound peptides from further reaction The linker holding the peptide and resin together is cleaved chemically to release the peptide The crude product is purified using either: reverse-phase high-performance liquid chromatography (HPLC) [9] [10] multicolumn countercurrent solvent gradient purification (MCSGP) which is utilised mainly in the case of longer peptides, due to accumulation of numerous minor byproducts that have similar properties to the desired peptide product