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The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay
10.1016/0003-2697(79)90738-3 56 OkadaK.FujisawaM
