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Nature 431: 11121117 Hu S, Balakrishnan A, Bok RA, Anderton B, Larson PEZ, Nelson SJ, Kurhanewicz J, Vigneron DB, Goga A (2011) 13Cpyruvate imaging reveals alterations in glycolysis that precede cMycinduced tumor formation and regression

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The frozen stock was thawed immediately before use and the reaction was initiated by adding an immobilized enzyme