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Briefly, Escherichia coli BL21 (DE3) cells (Novagen, Madison, WI, USA) transformed with the pET22b-Ts24GST construct, and were grown in 500 mL of LB medium supplemented with penicillin (100 g/mL)
Delaying care can make some conditions worse
LLY showed potent ACE inhibition (IC = 44.16 2.45 M) and remained stable across varied pH, moderate heat, metal ions, glucose, and salt
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