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Ethics declarations Competing interests KGW and VJNB are co-founders and shareholders of Aprea Therapeutics AB, a company that develops novel p53-based cancer therapy

This high background signal is a significant obstacle for diagnosing primary hepatocellular carcinoma, potentially limiting the utility to extrahepatic metastases unless structural modifications alter its excretion pathway (129)

Carrier protein is a matrix change and has to be recorded, and it is incompatible with some downstream analytical work, so the decision belongs in the protocol rather than in a footnote

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Furthermore, the universal Pan DR Epitope (PADRE) sequence (AKFVAAWTLKAAA), which effectively stimulates CD4+ responses, was incorporated after the M