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Elevated glucose levels impair white blood cell function, reduce bacterial killing capacity, and in some cases create glucose-rich environments that promote bacterial growth

Each assay plate contained a group of duplicates that were acquired by combining roughly 10 l from the first 76 samples in the study (QC pool duplicates) to allow for appropriate inter-plate abundance scaling based specifically on this cohort of samples ( n = 24 across all plates)

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When both receptors are activated simultaneously, the appetite-suppressing and metabolic effects appear to be additive in ways that single-receptor activation doesn't fully replicate