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Methylfolate is required in the activation of tetrahydrobiopterin (i.e., BH4), which is a necessary cofactor for both tryptophan hydroxylase and tyrosine hydroxylase, enzymes required in the synthesis of serotonin and norepinephrine/dopamine, respectively (Figure 1)

Certificate of Analysis is being prepared for this lot

J.de Aguiar VallimT

A single colony was inoculated into 100 ml of LB medium containing the appropriate antibiotics and incubated at 37 C overnight

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