48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min
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examined the effects of a dietary supplement called TOSLA with collagen, vitamin C, and hyaluronic acid (HA) on skin density, texture, and other parameters in a randomized, double-blind, placebo-controlled trial over 16 weeks in Slovenia [53]
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