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They isolated the protein responsible, then synthesized the active 15-amino-acid fragment

While melatonin isnt for ADHD symptoms per se, indirect benefits are likely: better mood, improved morning alertness, and possibly less need for high stimulant doses if the child is well-rested

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Cells were detached with EDTA, resuspended in HBSS, and furimazine (Promega) was added at a 1:50 dilution from the manufacturers pre-prepared stock