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After 4 weeks of D-galactose injections, the D-Gal group mice received intraperitoneal injections of PBS every 2 days for 4 weeks, followed by tissue collection

Sermorelin is a synthetic version of the naturally occurring growth hormone-releasing hormone (GHRH), a naturally-occuring peptide that tells your pituitary gland to release more growth hormone (2)

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After 12 h of incubation cells were washed by PBS, the cells adhering to the lower membrane of the inserts was counted after staining with 0.1% crystal violet