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To induce lung inflammation, the mice were anesthetized and were administered an intranasal instillation of a mixture containing LPS (5 mg/kg) and papain (10 units/mouse) dissolved in sterile PBS, with sample sizes of n = 2 and n = 3 for the two independent experiments

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Alanine is transferred to the circulation by many tissues, but mainly by skeletal muscle, in which alanine is formed from pyruvate at a rate proportional to intracellular pyruvate levels

Furthermore, the use of an iron chelator reduced cigarette smoke exposure (CSE)-induced EMT mitochondrial malfunction and cell death in lung epithelial cells

Environ Toxicol Pharmacol 74:103312