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Key Takeaways IGF-1 LR3 must be reconstituted with bacteriostatic water (0.9% benzyl alcohol) using slow-wall injection technique at 1mL per 2030 seconds to prevent protein denaturation from mechanical shear stress

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siRNA transfections were performed using Lipofectamine 2000 and ON-TARGETplus Human SMARTpool siRNAs (Dharmacon) against TYMS and a scrambled control pool, according to the manufacturers instructions

16th International p53 Workshop, 2014

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