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In MV4-11 cells, TMQ0153 treatment reduced total GSH (46.0%), GSH (24.8%), and the GSH/GSSG ratio (5.7%) within 30 min compared with the control (Fig

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Immunoblotting Protein samples were separated by SDS-PAGE, transferred to PVDF membrane, and probed with following antibodies: HNF4 (Santa Cruz, sc-374229, clone H-1, Lot B2417, 1:500), BHMT (Santa Cruz, sc-390299, clone H-7, Lot B1913, 1:500), CBS (Santa Cruz, sc-133154, clone B-4, Lot D0918, 1:1000), CDO1 (ThermoFisher Scientific, PA5-38005, Lot UD2757664A, 1:1000), MAT1A (Abcam, ab129176, Lot GR91375-9, 1:1000), CTH (Proteintech Group, 12217-1-AP, Lot 00076987, 1:1000), Puromycin (Millipore Sigma, MABE343, clone 12D10, Lot 3379285, 1:1000), Actin (Millipore Sigma, MAB1501, clone C4, Lot 3132961, 1:10,000), HRP-conjugated alpha Tubulin (Proteintech, HPR-66031, mouse monoclonal, Lot 21000018, 1:2000)

phytoactive Se generally exists as selenomethionine, which is biotransformed to synthesize specific selenoproteins and is not directly toxic even at a high doses (109)

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