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Tryptic peptides were separated by reversed-phase chromatography with a nanoflow high-performance liquid chromatography (HPLC) (Easy nLC II, Thermo Fisher Scientific) system on an EASY-column (10 cm, 75 m, C18) using gradient elution [developed from 5% (v/v) acetonitrile, 0.1% (v/v) formic acid to 90% (v/v) acetonitrile, 0.1% (v/v) formic acid in water over 40 min at a flow rate of 300 nl/min]

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This can be especially effective for persons who have been exposed to high levels of toxins

Personal/Family History: A history of thyroid disease makes screening more important